Mohammad Reza Nikravesh
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Title: Dysregulation Collagen IV and laminin α5 in seminiferous tubules of diabetic mice
Biography
Biography: Mohammad Reza Nikravesh
Abstract
Introduction: Diabetes is associated with reproductive impairment on the male reproductive system and causes complications such as testosterone decreasing and infertility. Extracellular matrix (ECM) molecules are involved in testicular development and spermatogenesis, laminin and collagen IV are key proteins in seminiferous tubule basement membrane (STBM) which can play an important role in spermatogenesis. In the present study was to investigate the effect of experimental diabetes on collagen IV and laminin α5 expression in mice testis.
Materials and methods: 40 Mice C57BL/6 were divided randomly into 4 groups: 1) treated mice with 50 mg/kg Streptozotocin (STZ) intraperitoneally for 5 days continuously, 2) treated mice with STZ + insulin, 3)control without intervention, 4)sham group that received citrate buffer. After 8 weeks, Left testes of all specimens were used for Real-Time PCR and right side were applied for immunohistochemical tracing methods.
Results: Immunoreactivity and gene expression of laminin α5 and collagen IV in the STBM showed a significant increase in diabetic mice in comparison to other groups (P<0.05).
Conclusion: Diabetes is likely to cause laminin α5 and collagen changes in the STBM of seminiferous tubules.