Mehdi Jalali
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Title: Assessment of hypothyroidism on laminin α5 and collagen IV disorder synthetize in mouse seminiferous tubules
Biography
Biography: Mehdi Jalali
Abstract
Introduction: There is evidence that extracellular matrix (ECM) components have profoundly impact on testis development, function and spermatogenesis. Hypothyroidism can negatively affect the basement membrane (BM) protein molecules and affect reproduction in the hypothyroid animals. The aim of this study was to investigate the effects of hypothyroidism on laminin α5 and collagen IV changes in BM seminiferous tubules.
Methods: 20 male mice were randomly divided into: 1) control group without any intervention, treated group with 0.05% propythiouracil (PTU) in drinking water for 2 months. After that, the left testicles were used for Real-Time PCR and the right specimens were used for immunohistochemistry staining methods.
Results: In this study, it was observed that hypothyroidism could increase significantly laminin α5 and Collagen IV mRNA expression. Moreover, Immunoreactivity had a significant increase in the PTU-treated in compared to control group.
Conclusion: These data indicate that hypothyroidism may induce abnormal changes in BM key proteins of seminiferous tubules such as collagen IV and laminin.